by bevoduz on Sat Mar 13, 2010 7:44 am
Not sure if your question was answered, but here's what I do with great results.
I use a stir plate to make my starters (if you don't make starters, I suggest looking into it, it makes harvesting yeast very easy, as well as improve your brews) I can my starter wort to simiplify this process, and have a powerpoint presentation I can send you outlining the steps if you want it.
I make a starter as usual (always make sure your starter gravity is on the low end to avoid mutating the yeast). When I pitch the yeast into the beer I got the starter going for, I leave some yeast in the flask, put it back on the stir plate with canned wort, and let them yeasties grow. Depending on how much I want to preserve depends on whether or not I add more wort when it's done fermenting. When it's ready, I pour off most of the wort, swirl the yeast with the remaining wort, and transfer it to the sanitized storage tubes I use. I use tubes just like white labs does, which are un-blown 2 liter plastic bottles. I bought a bunch of them and can sell you some if you need them, they're like $2 each or something and work great.
Then I just mark the tubes and put them in my fridge until I need them, at which time I create a starter and I'm ready to go. I've used the tubes as long as a year after creating them without any issues. I've even made tubes from that and used the yeast months later still without issues. One of the nice things about this is you always have yeast in case you or a friend needs it. Another nice thing is you can put a lot more yeast in the tubes than you'll get with a smack pack or white labs tube, so if you decide to brew at the last minute you have a much higher amount of yeast you're pitching with, and or a shorter time to get your starter to the level you need it.
I hope this helps!